3D PSII supercomplex; here
Jon Nield, Ph.D. - Scientist - a brief autobiography

For 25 years, discoveries of novel, photosynthetically active, protein macromolecular structures (20 to 40 nanometres in diameter) were elucidated by the structural biology techniques of Transmission Electron Microscopy (TEM) and single particle analysis. This body of work resulted in primary publications in Nature (3), Proceedings of the National Academy of Sciences USA (2), The Plant Cell (2), The Plant Journal and over 50 others in leading journals.

- 40+ invited talks were presented internationally and nationally. The protein structures observed involve a central photosystem 'reaction centre' coupled to peripheral light-harvesting components. These are now termed in the literature "photosynthetic supercomplexes". It is anticipated that renewable energy technologies, such as those involving solar capture and biohydrogen, will benefit from this knowledge of nature.

- awarded a Royal Society University Fellowship "Investigating Photosynthetic Complexes" in late 2001. This was extended to 2012 (0.69 FTE since 2009). The Fellowship's central remit was to probe more evolutionarily diverse organisms and mutants for light-harvesting photosynthetic proteins, and to drive forward a combinatorial approach of TEM and computer purification to overcome the structural heterogeneity often observed in light-sensitive samples. The Fellowship itself resulted in an output of over 25 primary papers

- further to this, separate structural breakthroughs were made for proteins involved in bacterial pathogenesis (PspA) and for antibiotic resistance (emrAB complex). Photosystem assembly (Ycf4 protein; ref: The Plant Cell, 2009) and red algal light harvesting (Lhcr proteins) were also reported upon. X-ray diffraction was used to probe a cyanobacterial light-harvesting protein, subsequently elucidated to a resolution of 1.45 in 2003 (the most highly resolved light harvesting protein at that time (for two years; with diffraction spots out to 1.1 ). Other projects used Atomic Force Microscopy to investigate molecular dynamics within membranes

- contributed to/designed/awarded 11 front covers of books and journals.

During the earliest stages of career (summer 1993), fortunate to isolate and fully characterise the first ever membrane-bound "photosynthetic supercomplex" (ref: PNAS, 1995) whilst working in the superb, vibrant, laboratory of James Barber at Imperial College London, together with Ben Hankamer (now at University of Queensland, Australia) and in collaboration with Egbert Boekema (Groningen) and Matthias Roegner (Bochum). This opened up a large field of international investigation surrounding photosystem/light-harvesting supercomplexes.

At that time, for his Ph.D. studies, he probed this (relatively huge, 33 nm diameter, elongated >1.2 megaDalton) PSII-LHCII supercomplex using 14 biochemical/biophysical techniques, gaining a Ph.D. in 1997, then followed this by its structural elucidation to 17 via cryo-TEM during two post-doctoral positions (refs: Nature/Nature Structural Biology). The use at that time of (relatively novel) vitrified samples permitted 3D structures to be calculated from a number of organisms (cyanobacteria, green algae and a diverse range of niche organisms and their mutants) by cryo-TEM and single particle image analysis (17-20 resolution).

- most satisfying to see the Nobel Prize for Chemistry (2017) awarded to 3 scientists who have played such an intrinsic role in bringing the field of cryo-EM into the state it is in, today. Indeed, it is the review paper of Dr. Henderson (Quart. Rev. Biophys. 1995) that played a big part of Jon's thinking during his thesis write-up, and in fact the motivation to apply for a PDRA position with Marin van Heel in 1997, and visits to the LMB in Cambridge in the late 1990's ... provided impetus to achieve early career contributions.

His lab engaged in many national/international collaborations with emphasis now on the mechanisms and complexes that assemble, repair and regulate these membrane proteins in the first instance, including the first 3D reconstruction of the PSII-affecting protease FtsH (ref: The Plant Cell, 2012). Aids in the revision of the IBID Press (Biology) textbooks (Hodder, ed. C. Talbot) as consultant, for the International Baccalaureate (2014-).

Refereed publications for many journals, both specialist and well known, and grants for the scientific community. Was an Associate Editor of Photochemical & Photobiological Sciences (01/2005 to 12/2009; www.rsc.org/pps). Concurrently (2005-2007) Staff Representative on Faculty and GM-safety committees, as well as organising a seminar series (26 lectures in 18 months), while sitting on Departmental IT provision committees. From 2012-2020 was Careers Liaison Officer role for Department's biology/biochemistry-degree UG/PG students (800+ individuals) and management committee member for QMUL's NanoVision TEM/SEM Centre (E1 4NS, London, UK).

Funding gratefully received over many years from The Royal Society, the BBSRC/UK government, The Biochemical Society and Japan's JST/CREST initiative, amongst others.

All original scientific content within this website is copyright of myself, the relevant authors, institutions and/or the journals that it is published in, as indicated. I am very grateful to have worked with so many different teams in over 150 different laboratories.

<updated 20th May 2022>

Jon Nield, 2000-2022